CHCHD5 Polyclonal antibody

CHCHD5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25711-1-AP

Synonyms

C2orf9, CHCHD5

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Validation Data Gallery

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  WB analysis using 25711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25711-1-AP (CHCHD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 25711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD5 antibody (25711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25711-1-AP targets CHCHD5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHCHD5 fusion protein Ag22503
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 5
• Calculated Molecular Weight: 110 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC004498
• Gene Symbol: CHCHD5
• Gene ID (NCBI): 84269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.