CHCHD4 Monoclonal antibody, PBS Only

CHCHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E4C6

Cat no : 66718-1-PBS

Synonyms

CHCHD4, FLJ31709, MIA40

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Validation Data Gallery

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  WB analysis using 66718-1-Ig (same clone as 66718-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66718-1-Ig (same clone as 66718-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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  IF Staining of SK-BR-3 using 66718-1-Ig (same clone as 66718-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using CHCHD4 antibody (66718-1-Ig, Clone: 3E4C6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

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Product Information

66718-1-PBS targets CHCHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHCHD4 fusion protein Ag15339
• Full Name: coiled-coil-helix-coiled-coil-helix domain containing 4
• Calculated Molecular Weight: 142 aa, 16 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC033775
• Gene Symbol: CHCHD4
• Gene ID (NCBI): 131474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).