CHAF1A Polyclonal antibody

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  WB analysis of Jurkat using 17037-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17037-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17037-1-AP

  IP result of anti-CHAF1A (IP:17037-1-AP, 4ug; Detection:17037-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human cervix using 17037-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 17037-1-AP (CHAF1A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervix using 17037-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 17037-1-AP (CHAF1A Antibody) at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

CHAF1A Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17037-1-AP

Publications(7)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ChIP, ELISA

Synonyms

CAF, CAF 1, CAF 1 subunit A, CAF I 150 kDa subunit, CAF I p150, CAF1, CAF1B, CAF1P150, CHAF1A, hp150, P150

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human cervix tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

17037-1-AP targets CHAF1A in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHAF1A fusion protein Ag10536
• Full Name: chromatin assembly factor 1, subunit A (p150)
• Calculated Molecular Weight: 956 aa, 107 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC067093
• Gene Symbol: CHAF1A
• Gene ID (NCBI): 10036
• RRID: AB_2291707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13111
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromatin assembly factor 1 (CAF1) is the only histone chaperone known to assemble histones H3 and H4 onto newly synthesized DNA both in vitro and in vivo [PMID:17065558]. The 938 amino acid multidomain p150 (CHAF1A) binds via its C-terminal third to p60, which is an essential step for nucleosome assembly because knocking down either subunit disrupts the activity [PMID:14519857]. In addition, CAF1 facilitates DNA synthesis depending on the binding of the N-terminal 31 residues of p150 to the proliferating cell nuclear antigen (PCNA), which acts as a sliding clamp to stimulate the processivity of DNA polymerase [PMID:10648606]. CHAF1A regulates the formation of heterochromatin in mammalian cells during replication and in plants it maintains the transcription of certain subsets of genes. Furthermore, CHAF1A exists in a chromatin-remodeling complex WINAC, which coactivates ligand-induced transactivation function of the vitamin D receptor [PMID:12837248]. CHAF1A protein exists some phosphorylation sites, which may affect its theoretical molecular weight when tested.And a 150 kDa band was recognized (PMID:27445493).

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; Reprogramming of the histone H3.3 landscape in the early mouse embryo.; Authors - Takashi Ishiuchi
human	ChIP	EMBO J; Histone chaperone CAF-1 promotes HIV-1 latency by leading the formation of phase-separated suppressive nuclear bodies.; Authors - Xiancai Ma ; KO Validated
human	IF	Cancers (Basel); Human Ccr4 and Caf1 Deadenylases Regulate Proliferation and Tumorigenicity of Human Gastric Cancer Cells via Modulating Cell Cycle Progression.; Authors - Xiao-Hui Song ; KD Validated
mouse	WB	J Biol Chem; The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.; Authors - Nasim A Begum
human	IHC	Front Mol Biosci; An integrated analysis of prognostic mRNA signature in early- and progressive-stage gastric adenocarcinoma; Authors - Xiaoling Hong
human	WB	Biomedicines; Co-Expression of Chromatin Assembly Factor 1 Subunit A and Proliferating Cell Nuclear Antigen Is a Prognostic Biomarker of Esophageal Cancer; Authors - Bing Wen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hasan (Verified Customer) (05-17-2023)

HeLa cells were pre-extracted for 5 mins then fixed with 4% PFA for 15 mins. Blocking with 5% FBS for 40 min and primary antibody incubation overnight.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HeLa cells

CHAF1A Antibody Immunofluorescence validation (1:300 dilution) in HeLa cells (Cat no:17037-1-AP)