CHAD Polyclonal antibody

CHAD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12963-1-AP

Synonyms

Cartilage leucine rich protein, CHAD, chondroadherin, SLRR4A

---

Validation Data Gallery

• 

  WB analysis of HeLa using 12963-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12963-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12963-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12963-1-AP targets CHAD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHAD fusion protein Ag4077
• Full Name: chondroadherin
• Calculated Molecular Weight: 359 aa, 40.5 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC036360
• Gene Symbol: CHAD
• Gene ID (NCBI): 1101
• RRID: AB_2244851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chondroadherin (CHAD), also known as cartilage leucine-rich protein, is a prominent noncollagenous extracellular protein in cartilage. It was first isolated from bovine cartilage. The primary structure of CHAD shows that it belongs to the family of leucine-rich repeat (LRR) proteins. It is expressed at high levels in certain zones of cartilage and also has been detected in bone, tendon, bone marrow, and chondrosarcoma cells. CHAD promotes attachment of chondrocytes, fibroblasts, and osteoblasts, mediated via the integrin alpha2beta1. It has also been shown to bind to collagen type II and both collagen type II and chondroadherin interact with integrin alpha2beta1. Besides, CHAD may play an important role in the regulation of chondrocyte growth and proliferation.

Publications

Species	Application	Title
human	WB	Reprod Sci; NFAT Overexpression Correlates with CA72-4 and Poor Prognosis of Ovarian Clear-Cell Carcinoma Subtype.; Authors - Bing Xin

Related Products

Collagen Type II Polyclonal antibody

15943-1-AP

CD41/Integrin alpha 2b Polyclonal antibody

24552-1-AP

Integrin Beta 1 Polyclonal antibody

12594-1-AP