CGREF1 Polyclonal antibody

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  WB analysis using 13323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13323-1-AP (CGREF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CGREF1 Polyclonal Antibody for WB, ELISA

Cat No. 13323-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CGRE1, CGR11, Cell growth regulator with EF hand domain protein 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13323-1-AP targets CGREF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CGREF1 fusion protein Ag3985
• Full Name: cell growth regulator with EF-hand domain 1
• Calculated Molecular Weight: 301 aa, 32 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC034764
• Gene Symbol: CGREF1
• Gene ID (NCBI): 10669
• RRID: AB_3669159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99674
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CGREF1, also known as CGR11, is predicted to enable calcium ion binding activity and to be involved in negative regulation of cell population proliferation. Human GREF1 protein has 4 clustered 17-amino acid repeats within the C terminus, suggesting a structure resembling 4 alpha-helical domains interrupted by distinct turns. Northern blot analysis of rat tissues demonstrated expression of CGR11 predominantly in the whole brain and kidney, with limited expression in heart, lung, liver, and skeletal muscle and no expression in spleen and testis.