CGGBP1 Polyclonal antibody

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  WB analysis using 10716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10716-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10716-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10716-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10716-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10716-1-AP (CGGBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CGGBP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10716-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, chIP, Dot Blot, ELISA

Synonyms

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

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Tested Applications

• Positive WB detected in: HeLa cells, mouse thymus tissue, K-562 cells, Raji cells, HL-60 cells, Jurkat cells, rat thymus tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Product Information

10716-1-AP targets CGGBP1 in WB, IHC, IF, IP, chIP, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CGGBP1 fusion protein Ag1129
• Full Name: CGG triplet repeat binding protein 1
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19-23 kDa
• GenBank Accession Number: BC005222
• Gene Symbol: CGGBP1
• Gene ID (NCBI): 8545
• RRID: AB_2079283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UFW8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

Publications

Species	Application	Title
human	IP	Cell Cycle; CGGBP1 phosphorylation constitutes a telomere-protection signal.; Authors - Umashankar Singh ; KD Validated
human	chIP	Cell Cycle; Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs.; Authors - Prasoon Agarwal
human	WB,IP	Epigenetics Chromatin; CGGBP1 regulates CTCF occupancy at repeats.; Authors - Divyesh Patel
human	IF	Exp Cell Res; CGGBP1 is a nuclear and midbody protein regulating abscission.; Authors - Singh Umashankar U
human	WB,Dot Blot	BMC Cancer; An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1.; Authors - Manthan Patel
human	WB	BMC Genet; CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity.; Authors - Manthan Patel