hCG Beta Polyclonal antibody

• Featured Product
• KD/KO Validated

hCG Beta Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11615-1-AP

Synonyms

CG beta, CGB, CGB3, CGB5, CGB7, CGB8, hCG, hCGB

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Validation Data Gallery

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  IHC staining of human placenta using 11615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11615-1-AP (hCG beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 11615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11615-1-AP (hCG beta antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 11615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11615-1-AP (hCG beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 11615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11615-1-AP (hCG beta antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 11615-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11615-1-AP (hCG beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11615-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11615-1-AP (hCG beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human placenta tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

11615-1-AP targets hCG Beta in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: hCG Beta fusion protein Ag2191
• Full Name: CG beta
• Calculated Molecular Weight: 165 aa, 18 kDa
• GenBank Accession Number: BC022796
• Gene Symbol: hCG Beta
• Gene ID (NCBI): 1082
• RRID: AB_2877783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CGB is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of CG. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

Publications

Species	Application	Title
human	WB,IHC	iScience; Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitus; Authors - Jie Ning
human	IF	EBioMedicine; Molecular mechanisms underlying altered neurobehavioural development of female offspring of mothers with polycystic ovary syndrome: FOS-mediated regulation of neurotrophins in placenta.; Authors - Fangfang Wang
human	IF	Cell Signal; Hypermethylation and low expression of FOXM1 predisposes women to unexplained recurrent miscarriage by impairing trophoblast stem cell proliferation; Authors - Youqing Jiang
human	WB	Mol Cell Endocrinol; Activation of G protein-coupled estrogen receptor stimulates placental human chorionic gonadotropin expression through PKA-CREB signaling; Authors - Sizhu Yang
human	WB	Biomolecules; Calnexin Is Involved in Forskolin-Induced Syncytialization in Cytotrophoblast Model BeWo Cells; Authors - Hitomi Matsukawa
human	WB,IF	Mol Hum Reprod; The roles of ADAMDEC1 in trophoblast differentiation during normal pregnancy and preeclampsia.; Authors - Zhuo-Hang Li