CFTR Polyclonal antibody
CFTR Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 20738-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lung tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
20738-1-AP targets CFTR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) |
| Calculated Molecular Weight | 168 kDa |
| GenBank Accession Number | NM_000492 |
| Gene Symbol | CFTR |
| Gene ID (NCBI) | 1080 |
| RRID | AB_2722558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the human cytogenetic location of the CFTR gene?
7q31.2
What is the predicted molecular weight of CFTR?
168 kDa
Where is CFTR expressed?
CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.
What is the ion selectivity of CFTR?
CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.
What is the process of CFTR trafficking?
The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.
What are the separate domains of CFTR?
CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.
What regulates CFTR?
CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.
What is the role of CFTR?
CFTR functions as a channel for anions, regulating water and ionic homeostasis.
What mutations in CFTR leads to protein degradation?
Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.
How does CFTR differ from other ATP-binding cassette proteins?
Unlike other proteins in the ABC family, CFTR functions as an ion channel.
PMIDs: 24513531, 24534272, 22966013, 22709980
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CFTR antibody 20738-1-AP | Download protocol |
| IF protocol for CFTR antibody 20738-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. | ||
PLoS One Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet. | ||
J Exp Clin Cancer Res Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer | ||
Int Immunopharmacol A novel transcriptomic signature associated with lymphovascular invasion predicts clinical outcomes, tumor microenvironment, and therapeutic response in lung adenocarcinoma | ||
Biochem Biophys Res Commun Dapagliflozin administration to a mouse model of type 2 diabetes induces DNA methylation and gene expression changes in pancreatic islets | ||
Asian J Androl The role of SLC26A8 homozygous variants in male infertility and flagellum abnormalities |
