CFHR3 Polyclonal antibody

CFHR3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16583-1-AP

Synonyms

CFHL3, CFHR3, complement factor H related 3, DOWN16, FHR 3, FHR3, H factor like protein 3, HLF4

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Validation Data Gallery

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  WB analysis using 16583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16583-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16583-1-AP

  IP result of anti-CFHR3 (IP:16583-1-AP, 4ug; Detection:16583-1-AP 1:300) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver using 16583-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16583-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human heart tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

16583-1-AP targets CFHR3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CFHR3 fusion protein Ag9963
• Full Name: complement factor H-related 3
• Calculated Molecular Weight: 330 aa, 37 kDa
• Observed Molecular Weight: 45-56 kDa
• GenBank Accession Number: BC058009
• Gene Symbol: CFHR3
• Gene ID (NCBI): 10878
• RRID: AB_2080151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. Deletion of CFHR1 and CFHR3 genes was found to be associated with decreased risk of age-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).

Publications

Species	Application	Title
human	WB	Clin Cancer Res; Complement regulatory proteins CFHR1 and CFHR3 and patient response to anti-CD20 monoclonal antibody therapy.; Authors - Laura M Rogers
human	WB	Sci Rep; A haplotype in CFH family genes confers high risk of rare glomerular nephropathies.; Authors - Yin Ding
human	IF	Exp Eye Res; Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration.; Authors - Swati Arya
human	WB	PLoS One; A novel quantitative hemolytic assay coupled with restriction fragment length polymorphisms analysis enabled early diagnosis of atypical hemolytic uremic syndrome and identified unique predisposing mutations in Japan.; Authors - Yoko Yoshida
human	WB,IHC	Bioengineered; A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.; Authors - Zhirui Zeng
human	WB	Mol Med Rep; Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis.; Authors - Hong Liu

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