Complement factor B Polyclonal antibody

Complement factor B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 10170-1-AP

Synonyms

CFB, Complement factor B Ba fragment, CFAB, C3/C5 convertase, BFD

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Validation Data Gallery

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  WB analysis using 10170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 10170-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 10170-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 10170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma, human blood, rat plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10170-1-AP targets Complement factor B in WB, IHC, ELISA, IF applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Complement factor B fusion protein Ag0226
• Full Name: complement factor B
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 93-100 kDa, 60-69 kDa
• GenBank Accession Number: BC007990
• Gene Symbol: Complement factor B
• Gene ID (NCBI): 629
• RRID: AB_2082384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

Publications

Species	Application	Title
human,mouse	IF	Nat Commun; The classical pathway triggers pathogenic complement activation in membranous nephropathy; Authors - Larissa Seifert
human	WB	J Cell Physiol; IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.; Authors - Xinxuan Cheng
human	WB,IHC,IF	Cell Oncol (Dordr); Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.; Authors - Reiri Shimazaki
human	WB	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
human,mouse	WB	Nephrol Dial Transplant; Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation.; Authors - Ming Wu
	WB	Chem Res Toxicol; 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.; Authors - Mikhail Linetsky

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

mark (Verified Customer) (03-30-2020)

worked with colourmetric immunohistochemistry

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: E13.5 to E18.5 embryo

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