Centrin 1 Monoclonal antibody

Centrin 1 Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8D10

Cat no : 68433-1-Ig

Synonyms

Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1

---

Validation Data Gallery

• 

  IF Staining of hTERT-RPE1 using 68433-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of ARPE-19 using 68433-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 68433-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells, ARPE-19 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68433-1-Ig targets Centrin 1 in IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Centrin 1 fusion protein Ag3529
• Full Name: centrin, EF-hand protein, 1
• Calculated Molecular Weight: 172 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC029515
• Gene Symbol: Centrin 1
• Gene ID (NCBI): 1068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.