CES3 Polyclonal antibody

CES3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14587-1-AP

Synonyms

UNQ869/PRO1887, ES31, EC:3.1.1.1, Carboxylesterase 3

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Validation Data Gallery

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  WB analysis using 14587-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14587-1-AP (CES3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 14587-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14587-1-AP (CES3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14587-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14587-1-AP (CES3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14587-1-AP targets CES3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CES3 fusion protein Ag6113
• Full Name: carboxylesterase 3
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 60-62 kDa
• GenBank Accession Number: BC053670
• Gene Symbol: CES3
• Gene ID (NCBI): 23491
• RRID: AB_2275992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CES3 is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is expressed in liver, colon and small intestine. This antibody recognizes both isoforms with MW 60-62 kDa and 24 kDa.

Publications

Species	Application	Title
mouse	WB	J Proteome Res; Profiling impaired hepatic endoplasmic reticulum glycosylation as a consequence of ethanol ingestion.; Authors - Galligan James J JJ
human	WB	Environ Toxicol Pharmacol; Formaldehyde alters triglyceride synthesis and very low-density lipoprotein secretion in a time-dependent manner.; Authors - Jianying Bai