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WB analysis of U-937 using 67079-1-Ig (same clone as 67079-1-PBS)
U-937 cells were subjected to SDS PAGE followed by western blot with 67079-1-Ig (CES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.
WB analysis of THP-1 using 67079-1-Ig (same clone as 67079-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 67079-1-Ig (CES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67079-1-Ig (same clone as 67079-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67079-1-Ig (same clone as 67079-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67079-1-Ig (same clone as 67079-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67079-1-Ig (CES1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

CES1 Monoclonal antibody, PBS Only

CES1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67079-1-PBS

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67079-1-PBS targets CES1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CES1 fusion protein Ag10647 Predict reactive species
Full Name	carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Calculated Molecular Weight	566 aa, 62 kDa
Observed Molecular Weight	60-63 kDa
GenBank Accession Number	BC012418
Gene Symbol	CES1
Gene ID (NCBI)	1066
RRID	AB_2882387
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P23141
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).