CES1 Polyclonal antibody, PBS Only

CES1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29041-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 29041-1-AP (same clone as 29041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29041-1-AP (same clone as 29041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29041-1-AP (CES1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 29041-1-AP (same clone as 29041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29041-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 29041-1-AP (same clone as 29041-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 29041-1-AP (same clone as 29041-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 29041-1-AP (same clone as 29041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CES1 antibody (29041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29041-1-PBS in a different storage buffer formulation.

---

Product Information

29041-1-PBS targets CES1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CES1 fusion protein Ag30534
• Full Name: carboxylesterase 1 (monocyte/macrophage serine esterase 1)
• Calculated Molecular Weight: 566 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC012418
• Gene Symbol: CES1
• Gene ID (NCBI): 1066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Human carboxylesterase 1 (hCE1 or CES1) is a serine esterase that hydrolyzes a variety of endogenous and exogenous substrates. CES1 contributes to approximately 90% of hepatic hydrolytic activity in human livers (PMID: 27895113). Variation in CES1 has the ability to affect the metabolism of methylphenidate (PMID: 28087982). CES1 has 567 amino acids and a theoretical molecular mass of 62 kDa.