CEP97 Polyclonal antibody

CEP97 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, Canine and More (2)





Cat no : 22050-1-AP


2810403B08Rik, centrosomal protein 97kDa, Centrosomal protein of 97 kDa, CEP97, LRRIQ2

Tested Applications

Positive WB detected inHL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22050-1-AP is CEP97 Fusion Protein expressed in E. coli.

Tested Reactivity human, Canine
Cited Reactivityhuman, mouse, Drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP97 fusion protein Ag16995
Full Name centrosomal protein 97kDa
Calculated Molecular Weight 865 aa, 97 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC041085
Gene Symbol CEP97
Gene ID (NCBI) 79598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.


Product Specific Protocols
WB protocol for CEP97 antibody 22050-1-APDownload protocol
IHC protocol for CEP97 antibody 22050-1-APDownload protocol
IF protocol for CEP97 antibody 22050-1-APDownload protocol
IP protocol for CEP97 antibody 22050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu

Nat Commun

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Authors - Ning Huang

J Cell Biol

Centrin2 regulates CP110 removal in primary cilium formation.

Authors - Suzanna L Prosser

Curr Biol

Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila.

Authors - Jeroen Dobbelaere

Curr Biol

Mother Centriole Distal Appendages Mediate Centrosome Docking at the Immunological Synapse and Reveal Mechanistic Parallels with Ciliogenesis.

Authors - Jane C Stinchcombe

Am J Hum Genet

Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome.

Authors - Laura Bozal-Basterra