• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CEP89, CCDC123 Polyclonal antibody, PBS Only

CEP89, CCDC123 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30165-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using CEP89, CCDC123 Polyclonal antibody (30165-1-AP)

    WB analysis of A549 using 30165-1-AP (same clone as 30165-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 30165-1-AP (CCDC123 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30165-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP89, CCDC123 Polyclonal antibody (30165-1-AP)

    IF Staining of hTERT-RPE1 using 30165-1-AP (same clone as 30165-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CCDC123 antibody (30165-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30165-1-PBS in a different storage buffer formulation.

Product Information

30165-1-PBS targets CEP89, CCDC123 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP89, CCDC123 fusion protein Ag32789
Full Name coiled-coil domain containing 123
Calculated Molecular Weight 783 aa, 90 kDa
Observed Molecular Weight 85-100 kDa
GenBank Accession NumberBC136328
Gene Symbol CEP89
Gene ID (NCBI) 84902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCDC123(as known as CEP123), also named as CEP89, is a new player in the process of primary ciliogenesis and it also plays a role in mitochondrial metabolism where it may modulate complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with Cep290(PMID:23575228, 23789104, 23348840). 24002-1-AP antibody recognizes all of CEP123 isoforms.