CEP83 Polyclonal antibody, PBS Only

CEP83 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 26013-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 26013-1-AP (same clone as 26013-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26013-1-AP (CEP83 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

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Product Information

26013-1-PBS targets CEP83 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP83 fusion protein Ag22826
• Full Name: coiled-coil domain containing 41
• Calculated Molecular Weight: 693 aa, 82 kDa
• Observed Molecular Weight: 83 kDa, 68 kDa
• GenBank Accession Number: BC125087
• Gene Symbol: CEP83
• Gene ID (NCBI): 51134
• RRID: AB_2880334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CEP83 (centrosomal protein, 83 kDa), also known as CCDC41 (coiled-coil domain containing 41), is required for ciliary vesicle docking to the mother centriole. It is a key component of the centriolar distal appendages. CCDC41 may collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly (PMID: 23530209). Alternative splicing results in transcript variants encoding two isoforms with calculated molecular weights of 83 kDa and 68 kDa, respectively.