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CEP72 Polyclonal antibody
CEP72 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 19928-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse testis tissue |
Positive IP detected in | HeLa cells |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 3 publications below |
IF | See 6 publications below |
IP | See 1 publications below |
Product Information
The immunogen of 19928-1-AP is CEP72 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CEP72 fusion protein Ag13905 |
Full Name | centrosomal protein 72kDa |
Calculated molecular weight | 646 aa, 72 kDa |
Observed molecular weight | 72 kDa |
GenBank accession number | BC001750 |
Gene symbol | CEP72 |
Gene ID (NCBI) | 55722 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CEP72 antibody 19928-1-AP | Download protocol |
IF protocol for CEP72 antibody 19928-1-AP | Download protocol |
IP protocol for CEP72 antibody 19928-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Elife Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
| ||
EMBO Rep Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy. | ||
J Cell Sci Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking. | ||
Sci Rep Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.
| ||
J Biol Chem Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131). | ||
BMC Biol CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72 |