CEP72 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP72 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19928-1-AP

Synonyms

centrosomal protein 72kDa, Centrosomal protein of 72 kDa, CEP72, KIAA1519

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Validation Data Gallery

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  WB analysis of HEK-293 using 19928-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19928-1-AP (CEP72 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19928-1-AP (CEP72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19928-1-AP

  IP result of anti-CEP72 (IP:19928-1-AP, 4ug; Detection:19928-1-AP 1:1000) with HeLa cells lysate 520ug.

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  IF Staining of HeLa using 19928-1-AP

  Immunofluorescent analysis of Hela cells, using CEP72 antibody 19928-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 19928-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP72 antibody (19928-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HEK-293 cells, rat testis tissue
• Positive IP detected in: HeLa cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

19928-1-AP targets CEP72 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP72 fusion protein Ag13905
• Full Name: centrosomal protein 72kDa
• Calculated Molecular Weight: 646 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC001750
• Gene Symbol: CEP72
• Gene ID (NCBI): 55722
• RRID: AB_10638607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IP, IF	Elife; Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.; Authors - Andrew Kodani ; KD Validated
human	IF	EMBO Rep; Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.; Authors - Andrea Martello
human	IF	J Cell Sci; Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.; Authors - Deniz Conkar
human	WB	Sci Rep; Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.; Authors - Deniz Conkar ; KD Validated
human	IF	J Biol Chem; Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131).; Authors - Ryan A Denu
human	WB,IF	BMC Biol; CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72; Authors - Zhenguang Ying