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  • KD/KO Validated

CEP72 Polyclonal antibody

CEP72 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 19928-1-AP

Synonyms

centrosomal protein 72kDa, Centrosomal protein of 72 kDa, CEP72, KIAA1519



Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IP detected inHeLa cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19928-1-AP is CEP72 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP72 fusion protein Ag13905
Full Name centrosomal protein 72kDa
Calculated molecular weight 646 aa, 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC001750
Gene symbol CEP72
Gene ID (NCBI) 55722
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CEP72 antibody 19928-1-APDownload protocol
IF protocol for CEP72 antibody 19928-1-APDownload protocol
IP protocol for CEP72 antibody 19928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IP, IF

Elife

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Authors - Andrew Kodani
  • KD Validated
humanIF

EMBO Rep

Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.

Authors - Andrea Martello
humanIF

J Cell Sci

Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.

Authors - Deniz Conkar
humanWB

Sci Rep

Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.

Authors - Deniz Conkar
  • KD Validated
humanIF

J Biol Chem

Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131).

Authors - Ryan A Denu
humanWB,IF

BMC Biol

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72

Authors - Zhenguang Ying