CEP70 Polyclonal antibody

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  WB analysis of human placenta using 16280-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16280-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16280-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16280-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP70 antibody (16280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CEP70 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16280-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

p10-binding protein, BITE

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse testis tissue, PC-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16280-1-AP targets CEP70 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP70 fusion protein Ag9365
• Full Name: centrosomal protein 70kDa
• Calculated Molecular Weight: 597 aa, 70 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC016050
• Gene Symbol: CEP70
• Gene ID (NCBI): 80321
• RRID: AB_2077083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NHQ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP70, also named as Centrosomal protein of 70 kDa, is a 597 amino acid protein, which directly interacts with tubulin-gamma; this interaction determines centrosomal localization. CEP70 plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, CEP70 is required for the organization and orientation of the mitotic spindle. CEP70 exists some isoforms with MW 70, 67, 65 and 25 kDa.

Publications

Species	Application	Title
human,mouse	IF	Front Endocrinol (Lausanne); Identification of biallelic variations of CEP70 in patients with male infertility; Authors - Tiechao Ruan
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang
human	WB	Cells; Co-Inhibition of tGLI1 and GP130 Using FDA-Approved Ketoconazole and Bazedoxifene Is Synergistic Against the Growth and Metastasis of HER2-Enriched and Triple-Negative Breast Cancers; Authors - Sara Manore