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  • Featured Product
  • KD/KO Validated

CEP55 Monoclonal antibody, PBS Only

CEP55 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11F7

Cat no : 67460-1-PBS

Synonyms

Up-regulated in colon cancer 6, Up regulated in colon cancer 6, Centrosomal protein of 55 kDa, centrosomal protein 55kDa, C10orf3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CEP55 Monoclonal antibody (67460-1-Ig)

    WB analysis of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    WB result of CEP55 antibody (67460-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CEP55 Monoclonal antibody (67460-1-Ig)

    WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CEP55 Monoclonal antibody (67460-1-Ig)

    WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using CEP55 Monoclonal antibody (67460-1-Ig)

    IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using CEP55 Monoclonal antibody (67460-1-Ig)

    IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP55 Monoclonal antibody (67460-1-Ig)

    IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP55 Monoclonal antibody (67460-1-Ig)

    IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP55 antibody (67460-1-Ig, Clone: 2D11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEP55 Monoclonal antibody (67460-1-Ig)

    IF Staining of HepG2 using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

Product Information

67460-1-PBS targets CEP55 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP55 fusion protein Ag29995
Full Name centrosomal protein 55kDa
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 47-55 kDa
GenBank Accession NumberBC008947
Gene Symbol CEP55
Gene ID (NCBI) 55165
RRIDAB_2882690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.