CEP55 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP55 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 23891-1-AP

Synonyms

C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, Up regulated in colon cancer 6, URCC6

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Validation Data Gallery

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  WB analysis of HeLa using 23891-1-AP

  WB result of CEP55 antibody (23891-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells.

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  WB analysis using 23891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 23891-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 23891-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 23891-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 23891-1-AP

  IP result of anti-CEP55 (IP:23891-1-AP, 4ug; Detection:23891-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 23891-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23891-1-AP (CEP55 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 23891-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23891-1-AP (CEP55 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 23891-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CEP55 antibody (23891-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat testis tissue, human testis tissue, HeLa cells, mouse testis tissue, Jurkat cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

23891-1-AP targets CEP55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP55 fusion protein Ag20940
• Full Name: centrosomal protein 55kDa
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 47-55 kDa
• GenBank Accession Number: BC008947
• Gene Symbol: CEP55
• Gene ID (NCBI): 55165
• RRID: AB_2802169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

Publications

Species	Application	Title
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	WB,IP,IF	Cell Death Dis; iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.; Authors - Kun Gao ; KD Validated
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Front Oncol; USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.; Authors - Catherine M Nickson ; KD Validated
human	WB,IHC	Cells; CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.; Authors - Minjing Li ; KD Validated
human	IF	iScience; Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission.; Authors - Kangji Wang

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