CEP250,C-NAP1 Monoclonal antibody, PBS Only

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  WB analysis using 66814-1-Ig (same clone as 66814-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66814-1-Ig (same clone as 66814-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

CEP250,C-NAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66814-1-PBS

Clone No.2G12D12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250,C-NAP1, CNAP1

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Product Information

66814-1-PBS targets CEP250,C-NAP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CEP250,C-NAP1 fusion protein Ag5925
• Full Name: centrosomal protein 250kDa
• Calculated Molecular Weight: 281 kDa
• Observed Molecular Weight: 280 kDa
• GenBank Accession Number: BC001433
• Gene Symbol: CEP250/CNAP1
• Gene ID (NCBI): 11190
• RRID: AB_2882157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BV73
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.