CEP250,C-NAP1 Monoclonal antibody, PBS Only

CEP250,C-NAP1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G12D12

Cat no : 66814-1-PBS

Synonyms

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250,C-NAP1, CNAP1

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Validation Data Gallery

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  WB analysis using 66814-1-Ig (same clone as 66814-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66814-1-Ig (same clone as 66814-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66814-1-PBS targets CEP250,C-NAP1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CEP250,C-NAP1 fusion protein Ag5925
• Full Name: centrosomal protein 250kDa
• Calculated Molecular Weight: 281 kDa
• Observed Molecular Weight: 280 kDa
• GenBank Accession Number: BC001433
• Gene Symbol: CEP250
• Gene ID (NCBI): 11190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.