Validation Data Gallery
|Positive WB detected in||HEK-293 cells|
|Positive IP detected in||RPE1 cells|
|Positive IHC detected in||human colon tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells, HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22227-1-AP is CEP164 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, Canine|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CEP164 fusion protein Ag17570|
|Full Name||centrosomal protein 164kDa|
|Calculated molecular weight||1460 aa, 164 kDa|
|Observed molecular weight||164 kDa|
|GenBank accession number||BC000602|
|Gene ID (NCBI)||22897|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CEP164, also named as KIAA1052 or NPHP15, is a 1460 amino acid protein, which contains 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.
Cep128 associates with Odf2 to form the subdistal appendage of the centriole.
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.
Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.
Methods Mol Biol
Analyzing Centrioles and Cilia by Expansion Microscopy.
J Biol Chem
Separable roles for RanGTP in nuclear and ciliary trafficking of a kinesin-2 subunit
Expansion microscopy for the analysis of centrioles and cilia.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Pierrick (Verified Customer) (10-24-2019)
Great antibody for immunofluorescence on human cells.Works well in WB