CEP164 Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP164 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

63

Reviews (3)

Cat no : 22227-1-AP

Synonyms

KIAA1052, CEP 164, 3F2B7, NPHP15, centrosomal protein 164kDa

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Validation Data Gallery

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  WB analysis of HEK-293 using 22227-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22227-1-AP (CEP164 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of RPE1 cells using 22227-1-AP

  WB results of CEP164 (22227-1-AP, 1:500) with RPE1 cells by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.

• 

  IHC staining of human colon using 22227-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 22227-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22227-1-AP (CEP164 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 22227-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 22227-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 22227-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 22227-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP164 antibody (22227-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa cells using 22227-1-AP

  IF results of CEP164 (22227-1-AP, 1:1000) with HeLa cells (1.5% formaldehyde, 10 min RT) by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.

• 

  IF Staining of HeLa using 22227-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP164 antibody (22227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 22227-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed hTERT-RPE1 cells using CEP164 antibody (22227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 22227-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CEP164 antibody (22227-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-3 using 22227-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CEP164 antibody (22227-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 22227-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22227-1-AP (CEP164 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: RPE1 cells
• Positive IHC detected in: human colon tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A549 cells, PC-3 cells, MDCK cells, hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 51 publications below
• IP: See 1 publications below

Product Information

22227-1-AP targets CEP164 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP164 fusion protein Ag17570
• Full Name: centrosomal protein 164kDa
• Calculated Molecular Weight: 1460 aa, 164 kDa
• Observed Molecular Weight: 164 kDa
• GenBank Accession Number: BC000602
• Gene Symbol: CEP164
• Gene ID (NCBI): 22897
• RRID: AB_2651175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP164, also named as KIAA1052 or NPHP15, is a 1460 amino acid protein, which contains 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.

Publications

Species	Application	Title
mouse	IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	IF	Nat Commun; M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.; Authors - Ning Huang
		Nat Commun; Genetic link between renal birth defects and congenital heart disease.; Authors - Jovenal T San Agustin
human	WB	Nat Commun; DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.; Authors - Xiaowei Xu
human	IF	EMBO J; ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.; Authors - Lauren T Evans
mouse	IF	Curr Biol; Rab34 is necessary for early stages of intracellular ciliogenesis.; Authors - Michael W Stuck ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ludovic (Verified Customer) (02-27-2024)

A fabulous antibody to identify the distal appendage of the primary cilium. Never seen an antibody that works so well!

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human cell line

FH

Elisa (Verified Customer) (04-04-2023)

H9 cells stained for Hoechst (DNA marker, in green), Cep164 (mother centriole distal appendage marker, in green) and g-Tubulin (pericentriolar matrix marker, in magenta). H9 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:300 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: H9 cells

CEP164 Antibody Immunofluorescence validation (1:300 dilution) in H9 cells (Cat no:22227-1-AP)

FH

Pierrick (Verified Customer) (10-24-2019)

Great antibody for immunofluorescence on human cells.Works well in WB

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: CEP164 IF 1/1000 to 1/2000 and WB 1/1000
• Cell Tissue Type: RPE1, HEK290, HeLa Kyoto

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