CEP135 Monoclonal antibody

CEP135 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C4B7

Cat no : 68715-1-Ig

Synonyms

CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7

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Validation Data Gallery

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  WB analysis using 68715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68715-1-Ig (CEP135 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68715-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP135 antibody (68715-1-Ig, Clone: 3C4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, U2OS cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68715-1-Ig targets CEP135 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CEP135 fusion protein Ag19122
• Full Name: centrosomal protein 135kDa
• Calculated Molecular Weight: 1140 aa, 134 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC136535
• Gene Symbol: CEP135
• Gene ID (NCBI): 9662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.