CELSR2 Recombinant antibody

CELSR2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230319H5

Cat no : 83115-1-RR

Synonyms

EGFL2, EGF like protein 2, CDHF10, Cadherin family member 10, Cadherin EGF LAG seven-pass G-type receptor 2

---

Validation Data Gallery

• 

  IF Staining of SH-SY5Y using 83115-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CELSR2 antibody (83115-1-RR, Clone: 230319H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 83115-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CELSR2 Recombinant antibody (83115-1-RR, Clone:230319H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83115-1-RR targets CELSR2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
• Calculated Molecular Weight: 318 kDa
• GenBank Accession Number: NM_001408
• Gene Symbol: CELSR2
• Gene ID (NCBI): 1952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may be important in cell/cell signaling during nervous system formation.