CEBPZ Polyclonal antibody, PBS Only

CEBPZ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25612-1-PBS

Synonyms

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Validation Data Gallery

• 

  WB analysis of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 25612-1-AP (same clone as 25612-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25612-1-AP (CEBPZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

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Product Information

25612-1-PBS targets CEBPZ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEBPZ fusion protein Ag11003
• Full Name: CCAAT/enhancer binding protein (C/EBP), zeta
• Calculated Molecular Weight: 1054 aa, 121 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC034475
• Gene Symbol: CEBPZ
• Gene ID (NCBI): 10153
• RRID: AB_2880160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.