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CEBPB Monoclonal antibody, PBS Only

CEBPB Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B6E10

Cat no : 66649-1-PBS

Synonyms

C/EBP beta, CEBPB, CRP2, IL6DBP, LAP, Liver activator protein, NF IL6, Nuclear factor NF IL6, TCF 5, TCF5, Transcription factor 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CEBPB Monoclonal antibody (66649-1-Ig)

    WB analysis of HeLa using 66649-1-Ig (same clone as 66649-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66649-1-Ig (CEBPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using CEBPB Monoclonal antibody (66649-1-Ig)

    WB analysis of L02 using 66649-1-Ig (same clone as 66649-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66649-1-Ig (CEBPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CEBPB Monoclonal antibody (66649-1-Ig)

    WB analysis of HepG2 using 66649-1-Ig (same clone as 66649-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66649-1-Ig (CEBPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CEBPB Monoclonal antibody (66649-1-Ig)

    IHC staining of human lung cancer using 66649-1-Ig (same clone as 66649-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66649-1-Ig (CEBPB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66649-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66649-1-PBS targets CEBPB in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEBPB fusion protein Ag20073
Full Name CCAAT/enhancer binding protein (C/EBP), beta
Calculated Molecular Weight 345 aa, 36 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC007538
Gene Symbol CEBPB
Gene ID (NCBI) 1051
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation By similarity. Regulates the transcriptional induction of peroxisome proliferator-activated receptor gamma (PPARG). CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein. All major isoforms of CEBPB (38, 34, and 20 kDa) are expressed, with the 34 and 20kDa isoforms being more abundant in preovulatory follicles and further increased in corpora lutea (CL)(PMID:15647458).The truncated protein of 18 kDa (relative to the 30 kDa full-length protein that is known as LAP, or p30 CEBPb or liver-activating protein) lacks a transactivation domain,also known as LIP (p19 CEBPb or liver-inhibitory protein), can form homodimers or heterodimerize with other family members and, as it lacks the transactivation domain, can attenuate the transcriptional activation properties of the other isoforms.(10051447). Three variants of CEBPBs have been detected in many cell types: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20-kDa liver-enriched transcription-inhibitory protein (LIP). These variants are the result of an alternative translation initiation due to a leaky ribosomal scanning mechanism.(PMID:18820298).