CEBP Alpha/CEBPA Polyclonal antibody

CEBP Alpha/CEBPA Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 29388-1-AP

Synonyms

CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα, C/EBP alpha

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Validation Data Gallery

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  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBP Alpha/CEBPA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBP Alpha/CEBPA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 29388-1-AP

  IP result of anti-CEBP Alpha/CEBPA (IP:29388-1-AP, 4ug; Detection:29388-1-AP 1:4000) with THP-1 cells lysate 1520 ug.

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Tested Applications

• Positive WB detected in: BGC-823 cells, LNCaP cells, THP-1 cells, U-937 cells, K-562 cells, L02 cells, HL-60 cells
• Positive IP detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below

Product Information

29388-1-AP targets CEBP Alpha/CEBPA in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEBP Alpha/CEBPA fusion protein Ag29947
• Full Name: CCAAT/enhancer binding protein (C/EBP), alpha
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_004364
• Gene Symbol: CEBPA
• Gene ID (NCBI): 1050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).

Publications

Species	Application	Title
human,rat	WB	Front Pharmacol; Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism; Authors - Tian Li
	WB	J Virol; Dengue virus NS1 leads to downregulation of HNF4 alpha in liver cells resulting in a decrease in coagulation factors I, V, X, and XIII, contributing to coagulopathy; Authors - Sandeepan Das
mouse	WB	Pharmaceuticals (Basel); Potential Anti-Obesity Effect of Hazel Leaf Extract in Mice and Network Pharmacology of Selected Polyphenols; Authors - Jiarui Zhao
human	WB,IHC	Sci Rep; A comprehensive analysis of CEBPA on prognosis and function in uterine corpus endometrial carcinoma; Authors - Jiaxing Wang
human	WB	Genes Dis; MAFB-mediated CEBPA regulated human urothelium growth through Wnt/β-catenin signaling pathway; Authors - Zhenmin Liu
rat	WB	Mol Med; Ginsenoside Rb1 affects mitochondrial Ca2+ transport and inhibits fat deposition and fibrosis by regulating the wnt signaling pathway to treat rotator cuff tears via docking with SFRP1; Authors - Yuesong Yin