CEBP Alpha/CEBPA Polyclonal antibody

CEBP Alpha/CEBPA Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 29388-1-AP

Synonyms

CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα, C/EBP alpha

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Validation Data Gallery

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  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBP Alpha/CEBPA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBP Alpha/CEBPA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 29388-1-AP

  IP result of anti-CEBP Alpha/CEBPA (IP:29388-1-AP, 4ug; Detection:29388-1-AP 1:4000) with THP-1 cells lysate 1520 ug.

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Tested Applications

• Positive WB detected in: BGC-823 cells, LNCaP cells, THP-1 cells, U-937 cells, K-562 cells, L02 cells, HL-60 cells
• Positive IP detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

29388-1-AP targets CEBP Alpha/CEBPA in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEBP Alpha/CEBPA fusion protein Ag29947
• Full Name: CCAAT/enhancer binding protein (C/EBP), alpha
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_004364
• Gene Symbol: CEBPA
• Gene ID (NCBI): 1050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).

Publications

Species	Application	Title
human,rat	WB	Front Pharmacol; Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism; Authors - Tian Li
mouse	WB	J Agric Food Chem; In Vitro Comparative Analysis of the Effect and Structure-Based Influencing Factors of Flavonols on Lipid Accumulation; Authors - Beijun Ang
human	WB	Toxics; Tris (2-chloroisopropyl) phosphate and Tris (nonylphenyl) phosphite Promote Human Renal Cell Apoptosis through the ERK/CEPBA/Long Non-Coding RNA Cytoskeleton Regulator Axis; Authors - Hu Zhao ; KD Validated
mouse	WB	Cardiovasc Diabetol; miPEP31 alleviates Ang II-induced hypertension in mice by occupying Cebpα binding sites in the pri-miR-31 promoter; Authors - Xiangxiao Li
human	WB	Cell Biochem Biophys; Blockade of ITGA2/3/5 Promotes Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells; Authors - Ying Li
human	WB	Sci Rep; Polypeptide N-acetylgalactosaminyltransferase-15 regulates adipogenesis in human SGBS cells; Authors - Asuka Takahashi