CEACAM6 Polyclonal antibody

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  WB analysis using 30569-1-AP

  Untreated and PNGase F-treated lysates of A549 cells were subjected to SDS PAGE followed by western blot with 30569-1-AP (CEACAM6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

CEACAM6 Polyclonal Antibody for WB, ELISA

Cat No. 30569-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

CD66c, CEACAM6, CEAL, NCA, Normal cross reacting antigen

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Tested Applications

• Positive WB detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30569-1-AP targets CEACAM6 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 70-90 kDa, 37 kDa
• GenBank Accession Number: BC005008
• Gene Symbol: CEACAM6
• Gene ID (NCBI): 4680
• RRID: AB_3086364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P40199
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEACAM6, also known as CD66c, is a glycosylphosphatidylinositol (GPI)-linked cell surface protein that belongs to the CEACAM family of the immunoglobulin superfamily (PMID: 23903773). It is normally expressed on the surface of myeloid and epithelial surfaces and upregulated preferentially at the apical/luminal membranes of many tumors (PMID: 27595061; 35082925). CEACAM6 plays an important role in cell adhesion, proliferation, tumorigenesis, metastasis, and immunity (PMID: 31797958).