Anti-Human CEACAM3/6 (CD66c/d) Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98169-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CEACAM3/6 (CD66c/d) Rabbit Recombinant Antibody (98169-1-RR, Clone: 241254D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98169-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98169-1-RR against Human CEACAM3/6 (CD66c/d) were performed. The affinity constant is 2.19 nM.

CEACAM3/6 (CD66c/d) Recombinant Antibody for FC

Cat No. 98169-1-RR

Clone No.241254D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD66c, CEACAM3/CD66d, CEACAM6, CEACAM6/CD66c, CEACAM3

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes, FC receptor blocked human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98169-1-RR targets CEACAM3/6 (CD66c/d) in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 3
• Calculated Molecular Weight: 252 aa, 27 kDa
• GenBank Accession Number: BC106728
• Gene Symbol: CEACAM3
• Gene ID (NCBI): 1084
• RRID: AB_3672312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P40198
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily of cell adhesion molecules. CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in controlling cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM3, also named as CD66d and CGM1, is exclusively expressed on granulocytes and acts as the major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms (PMID: 18606569). CEACAM6, also known as CD66c, is normally expressed on the surface of myeloid and epithelial surfaces and upregulated preferentially at the apical/luminal membranes of many tumors (PMID: 27595061; 35082925). This antibody is raised against 35-155 aa of human CEACAM3/CD66d and can cross-react with CEACAM6/CD66c.