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CEACAM1/CD66a Recombinant antibody, PBS Only (Capture)

CEACAM1/CD66a Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240497B4

Cat no : 83524-3-PBS

Synonyms

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEACAM1/CD66a Recombinant antibody (83524-3-RR)

    WB analysis using 83524-3-RR (same clone as 83524-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CEACAM1/CD66a Recombinant antibody (83524-3-RR)

    IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CEACAM1/CD66a Recombinant antibody (83524-3-RR)

    IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00520-2

    Cytometric bead array standard curve of MP00520-2

    Cytometric bead array standard curve of MP00520-2, CEACAM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83524-3-PBS. Detection antibody: 83524-4-PBS. Standard: Eg0314. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00520-3

    Cytometric bead array standard curve of MP00520-3

    Cytometric bead array standard curve of MP00520-3, CEACAM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83524-3-PBS. Detection antibody: 83524-2-PBS. Standard: Eg0314. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83524-3-RR

    Affinity and Kinetic Characterization of 83524-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83524-3-RR against Human CEACAM1 were performed. The affinity constant is 3.58 nM.

Product Information

83524-3-PBS targets CEACAM1/CD66a as part of a matched antibody pair:

MP00520-2: 83524-3-PBS capture and 83524-4-PBS detection (validated in Cytometric bead array)

MP00520-3: 83524-3-PBS capture and 83524-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Calculated Molecular Weight 526 aa, 58 kDa
Observed Molecular Weight135-150 kDa
GenBank Accession NumberBC014473
Gene Symbol CEACAM1
Gene ID (NCBI) 634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).