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  • KD/KO Validated

CEA/CD66e Polyclonal antibody

CEA/CD66e Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10421-1-AP

Synonyms

CEA, Meconium antigen 100, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5



Tested Applications

Positive WB detected inHT-29 cells, MCF-7 cells, human saliva
Positive IHC detected inhuman colon cancer tissue, human appendicitis tissue, human stomach cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10421-1-AP targets CEA/CD66e in WB, IHC, IF-P, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEA/CD66e fusion protein Ag0672
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated Molecular Weight 77 kDa
Observed Molecular Weight180-200 kDa, 77 kDa
GenBank Accession NumberBC034671
Gene Symbol CEA
Gene ID (NCBI) 1048
RRIDAB_2244691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

Protocols

Product Specific Protocols
WB protocol for CEA/CD66e antibody 10421-1-APDownload protocol
IHC protocol for CEA/CD66e antibody 10421-1-APDownload protocol
IF protocol for CEA/CD66e antibody 10421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Res

Molecular signature of interleukin-22 in colon carcinoma cells and organoid models.

Authors - Ina Rudloff
humanWB,IHC,chIP

Int J Biol Sci

FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis.

Authors - Qingguo Li
  • KD Validated
humanIHC

Gene Ther

Lentivirus-mediated RASSF1A expression suppresses aggressive phenotypes of gastric cancer cells in vitro and in vivo.

Authors - P-H Zhou
humanIHC

World Neurosurg

Discovery of Aquaporin-1 and Aquaporin-4 Expression in an Intramedullary Spinal Cord Ependymal Cyst: Case Report.

Authors - Matthew J Shepard
humanWB

PLoS One

Correlation of alpha-1 antitrypsin levels and exosome associated neutrophil elastase endothelial injury in subjects with SARS-CoV2 infection

Authors - Jorge Lascano
mouseWB

Iran J Basic Med Sci

Preparation and application of patient-derived xenograft mice model of colorectal cancer

Authors - Yutao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Claire (Verified Customer) (09-30-2024)

A repeat order of an antibody previously used in colorectal adenocarcinoma research as this produced excellent images previously, we are now aiming to optimise this antibody for pancreatic work

  • Applications: Immunohistochemistry, Immunofluorescence
  • Cell Tissue Type: Colorectal adenocarcinoma / pancreatic carcinoma