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CEA Polyclonal antibody

CEA Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 10421-1-AP


Carcinoembryonic antigen, CD66e, CEA, CEACAM5, Meconium antigen 100

Tested Applications

Positive WB detected inHT-29 cells, MCF-7 cells, human saliva
Positive IHC detected inhuman colon cancer tissue, human appendicitis tissue, human stomach cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10421-1-AP targets CEA in WB, IF, IHC, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEA fusion protein Ag0672
Full Name carcinoembryonic antigen-related cell adhesion molecule 5
Calculated Molecular Weight 77 kDa
Observed Molecular Weight180-200 kDa, 77 kDa
GenBank Accession NumberBC034671
Gene Symbol CEA
Gene ID (NCBI) 1048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.


Product Specific Protocols
WB protocol for CEA antibody 10421-1-APDownload protocol
IHC protocol for CEA antibody 10421-1-APDownload protocol
IF protocol for CEA antibody 10421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Molecular signature of interleukin-22 in colon carcinoma cells and organoid models.

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FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis.

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Preparation and application of patient-derived xenograft mice model of colorectal cancer

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