CDX2 Polyclonal antibody

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55286-1-AP

Synonyms

CDX3, CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2

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Validation Data Gallery

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  WB analysis of COLO 320 using 55286-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55286-1-AP (CDX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 55286-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55286-1-AP (CDX2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55286-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55286-1-AP (CDX2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 55286-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (55286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IHC detected in: rat colon tissue, human colon cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55286-1-AP targets CDX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caudal type homeobox 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_001265
• Gene Symbol: CDX2
• Gene ID (NCBI): 1045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.