CDR2L Monoclonal antibody

• Featured Product
• KD/KO Validated

CDR2L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2E12D10

Cat no : 66791-1-Ig

Synonyms

CDR2L, HUMPPA, Paraneoplastic 62 kDa antigen

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Validation Data Gallery

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  WB analysis of U-251 using 66791-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66791-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66791-1-Ig

  WB result of CDR2L antibody (66791-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected HEK-293 cells.

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  WB analysis of HEK-293 using 66791-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66791-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66791-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66791-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66791-1-Ig (CDR2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 66791-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human cerebellum using 66791-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66791-1-Ig (CDR2L antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of HEK-293 using 66791-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CDR2L antibody (66791-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U-251 cells, HEK-293 cells, K-562 cells, human brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IP: See 1 publications below

Product Information

66791-1-Ig targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDR2L fusion protein Ag6139
• Full Name: cerebellar degeneration-related protein 2-like
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC047534
• Gene Symbol: CDR2L
• Gene ID (NCBI): 30850
• RRID: AB_2882135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm.

Publications

Species	Application	Title
human	IP	Ann Clin Transl Neurol; Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.; Authors - Ida Herdlevaer