CDR2L Polyclonal antibody

• Featured Product
• KD/KO Validated

CDR2L Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14563-1-AP

Synonyms

Paraneoplastic 62 kDa antigen, HUMPPA, Cerebellar degeneration-related protein 2-like

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Validation Data Gallery

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  WB analysis of K-562 using 14563-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14563-1-AP (CDR2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14563-1-AP

  WB result of CDR2L antibody (14563-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDR2L transfected SH-SY5Y cells.

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  IP experiment of SH-SY5Y using 14563-1-AP

  IP result of anti-CDR2L (IP:14563-1-AP, 3ug; Detection:14563-1-AP 1:500) with SH-SY5Y cells lysate 2500ug.

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  IHC staining of human cerebellum using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14563-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14563-1-AP (CDR2L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14563-1-AP

  Immunofluorescent analysis of HepG2 cells using 14563-1-AP (CDR2L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

14563-1-AP targets CDR2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDR2L fusion protein Ag6127
• Full Name: cerebellar degeneration-related protein 2-like
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC047534
• Gene Symbol: CDR2L
• Gene ID (NCBI): 30850
• RRID: AB_2076740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cerebellar degeneration-related protein 2-like(CDR2L), also named as HUMPPA or Paraneoplastic 62 kDa antigen, is a 465 amino acid protein, which belongs to the CDR2 family. CDR2L contains three potential coiled-coil regions. The functions of protein is so far not known. CDR2L is expressed in the brain and localizes in the cytoplasm. The predicted MW of this protein is 53 kDa.

Publications

Species	Application	Title
human	IP, WB	Acta Neuropathol; Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.; Authors - Manja Schubert
human	WB,IHC	Cancer Immunol Immunother; Cerebellar degeneration-related proteins 2 and 2-like are present in ovarian cancer in patients with and without Yo antibodies.; Authors - Margrethe Raspotnig
human	WB,IF,IP	Ann Clin Transl Neurol; Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.; Authors - Ida Herdlevaer
human,rat	WB,IHC	J Neuroimmunol; Expression of cerebellar degeneration-related proteins CDR2 and CDR2L in human and rat brain tissue.; Authors - Margrethe Raspotnig