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CDO1 Polyclonal antibody

CDO1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12589-1-AP

Synonyms

CDO, CDO I, CDO1, Cysteine dioxygenase type 1, cysteine dioxygenase, type I



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12589-1-AP targets CDO1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDO1 fusion protein Ag3294
Full Name cysteine dioxygenase, type I
Calculated Molecular Weight 200 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC024241
Gene Symbol CDO1
Gene ID (NCBI) 1036
RRIDAB_10638145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDO1(cysteine dioxygenase type 1) is also named as CDO and belongs to the cysteine dioxygenase family. It is an enzyme that adds molecular oxygen to the sulfur of cysteine, converting the thiol to a sulfinic acid known as cysteinesulfinic acid (3-sulfinoalanine) and CDO1 is one of the most highly regulated metabolic enzymes responding to diet(PMID:19011731). The expression of CDO can significantly decrease the level of intracellular cysteine and this reduction is also associated with a decrease in total glutathione levels(PMID:17327371). Cysteine dioxygenase (CDO) plays a critical role in the regulation of cellular cysteine concentration andmultiple forms of CDO (23 kDa, 25 kDa, and 68 kDa) have been claimed based upon separation and detection using SDS-PAGE/western blotting(PMID: 14752623).

Protocols

Product Specific Protocols
WB protocol for CDO1 antibody 12589-1-APDownload protocol
IHC protocol for CDO1 antibody 12589-1-APDownload protocol
IP protocol for CDO1 antibody 12589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Discov

Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells

Authors - Gang Ma
humanIHC

Int J Biol Sci

Ferroptosis-associated molecular classification characterized by distinct tumor microenvironment profiles in colorectal cancer.

Authors - Wenqin Luo
mouseWB

J Nutr Biochem

PMID: 24917045

Authors - Kerstin E Geillinger
ratWB

J Ethnopharmacol

Integration of metabolomics and transcriptomics to reveal anti-chronic myocardial ischemia mechanism of Gualou Xiebai decoction.

Authors - Fengyun Zhang
humanIHC

Ann Surg Oncol

Promoter DNA Hypermethylation of the Cysteine Dioxygenase 1 (CDO1) Gene in Intraductal Papillary Mucinous Neoplasm (IPMN).

Authors - Yoshiki Fujiyama
mouseWB

Cell Mol Neurobiol

Taurine Alleviates Chronic Social Defeat Stress-Induced Depression by Protecting Cortical Neurons from Dendritic Spine Loss.

Authors - Yuanyuan Zhu