CDK9 Recombinant antibody, PBS Only (Detector)

CDK9 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240600E3

Cat no : 83662-2-PBS

Synonyms

CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k

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Validation Data Gallery

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  WB analysis using 83662-2-RR (same clone as 83662-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83662-2-RR (CDK9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-2-RR, Clone: 240600E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83662-2-RR (same clone as 83662-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK9 Recombinant antibody (83662-2-RR, Clone:240600E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83662-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00630-2

  Cytometric bead array standard curve of MP00630-2, CDK9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83662-5-PBS. Detection antibody: 83662-2-PBS. Standard: Ag2318. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83662-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83662-2-RR against Human CDK9 were performed. The affinity constant is 0.305 nM.

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Product Information

83662-2-PBS targets CDK9 as part of a matched antibody pair:

MP00630-2: 83662-5-PBS capture and 83662-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDK9 fusion protein Ag2318
• Full Name: cyclin-dependent kinase 9
• Calculated Molecular Weight: 372 aa, 43 kDa
• Observed Molecular Weight: 38-42 kDa, 55 kDa
• GenBank Accession Number: BC001968
• Gene Symbol: CDK9
• Gene ID (NCBI): 1025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).