CDK7 Monoclonal antibody

CDK7 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E7B6

Cat no : 67889-1-Ig

Synonyms

39 kDa protein kinase, CAK, CAK1, CDK activating kinase, CDK7, CDKN7, Cell division protein kinase 7, cyclin dependent kinase 7, MO15, p39 Mo15, p39MO15, STK1

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Validation Data Gallery

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  WB analysis using 67889-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 67889-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67889-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67889-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67889-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A431 using 67889-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, U2OS cells, MCF-7 cells, Hela cells, Jurkat cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67889-1-Ig targets CDK7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDK7 fusion protein Ag25737
• Full Name: cyclin-dependent kinase 7
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC005298
• Gene Symbol: CDK7
• Gene ID (NCBI): 1022
• RRID: AB_2918645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.