CDK5RAP3 Polyclonal antibody

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  WB analysis using 11007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11007-1-AP

  WB result of CDK5RAP3 antibody (11007-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK5RAP3 transfected HEK-293 cells.

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  IHC staining of human stomach using 11007-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 11007-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 11007-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11007-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver using 11007-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CDK5RAP3 antibody (11007-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CDK5RAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 11007-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Synonyms

IC53, HSF27, HSF 27, CDK5 regulatory subunit-associated protein 3, CDK5 activator-binding protein C53

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human stomach tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

11007-1-AP targets CDK5RAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK5RAP3 fusion protein Ag1474
• Full Name: CDK5 regulatory subunit associated protein 3
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC009957
• Gene Symbol: CDK5RAP3
• Gene ID (NCBI): 80279
• RRID: AB_2076869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96JB5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

Publications

Species	Application	Title
mouse,bovine	WB	Int J Mol Sci; CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury.; Authors - Yangyang Shen ; KD Validated
human	WB	Autophagy; The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy; Authors - Jiangnan Liu