CDK4 Recombinant antibody, PBS Only

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  WB analysis using 83994-3-RR (same clone as 83994-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83994-3-RR (CDK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 83994-3-RR (same clone as 83994-3-PBS)

  IP result of anti-CDK4 (IP:83994-3-RR, 4ug; Detection:83994-3-RR 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 83994-3-RR (same clone as 83994-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83994-3-RR (CDK4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83994-3-RR (same clone as 83994-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (83994-3-RR, Clone: 241053F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

CDK4 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 83994-3-PBS

Clone No.241053F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4

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Product Information

83994-3-PBS targets CDK4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDK4 fusion protein Ag20538
• Full Name: cyclin-dependent kinase 4
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC010153
• Gene Symbol: CDK4
• Gene ID (NCBI): 1019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P11802
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1-to S-phase transition during the cell cycle, and its expression is primarily controlled at the transcriptional level(PMID:17253961). The CCND1-CDK4 axis is critical not only in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment, which are vital to sustain tumor outgrowth (PMID:21844184).