CDK4 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00897-1

  Sandwich ELISA standard curve of MP00897-1, Human CDK4 Recombinant Matched Antibody Pair - PBS only. 83994-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20538. 83994-1-PBS was HRP conjugated as the detection antibody. Range: 0.937-60 ng/mL.

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  Sample test of MP00897-1

  The mean CDK4 concentration was determined to be 257.14 ng/mL in HeLa cell extract based on a 2.00 mg/mL extract load.

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  Cytometric bead array standard curve of MP00897-1

  Cytometric bead array standard curve of MP00897-1, CDK4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83994-2-PBS. Detection antibody: 83994-1-PBS. Standard: Ag20538. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83994-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83994-2-PBS against Human CDK4 was performed. The affinity constant is 0.42 nM.

CDK4 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83994-2-PBS

Clone No.241053C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 4, cyclin dependent kinase 4, CMM3, Cell division protein kinase 4

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Product Information

83994-2-PBS targets CDK4 as part of a matched antibody pair:

MP00897-1: 83994-2-PBS capture and 83994-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDK4 fusion protein Ag20538
• Full Name: cyclin-dependent kinase 4
• Calculated Molecular Weight: 34 kDa
• GenBank Accession Number: BC010153
• Gene Symbol: CDK4
• Gene ID (NCBI): 1019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11802
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.