CDK2 Recombinant antibody, PBS Only (Capture/Detector)

CDK2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240580G11

Cat no : 83635-2-PBS

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

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Validation Data Gallery

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  WB analysis using 83635-2-RR (same clone as 83635-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83635-2-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83635-2-RR (same clone as 83635-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83635-2-RR (CDK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83635-2-RR (same clone as 83635-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (83635-2-RR, Clone: 240580G11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83635-2-RR (same clone as 83635-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-2-RR, Clone:240580G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00611-1

  Cytometric bead array standard curve of MP00611-1, CDK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83635-3-PBS. Detection antibody: 83635-2-PBS. Standard: Ag17133. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP00611-2

  Cytometric bead array standard curve of MP00611-2, CDK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83635-2-PBS. Detection antibody: 83635-1-PBS. Standard: Ag17133. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83635-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83635-2-RR against Human CDK2 were performed. The affinity constant is 3.23 nM.

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Product Information

83635-2-PBS targets CDK2 as part of a matched antibody pair:

MP00611-1: 83635-3-PBS capture and 83635-2-PBS detection (validated in Cytometric bead array)

MP00611-2: 83635-2-PBS capture and 83635-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CDK2 fusion protein Ag17133
• Full Name: cyclin-dependent kinase 2
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC003065
• Gene Symbol: CDK2
• Gene ID (NCBI): 1017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.