CDK10 Polyclonal antibody

CDK10 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17182-1-AP

Synonyms

CDK10, cyclin dependent kinase 10, PISSLRE

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Validation Data Gallery

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  IHC staining of mouse brain using 17182-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17182-1-AP (CDK10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17182-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17182-1-AP (CDK10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17182-1-AP targets CDK10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK10 fusion protein Ag10935
• Full Name: cyclin-dependent kinase 10
• Calculated Molecular Weight: 283 aa, 32 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC017342
• Gene Symbol: CDK10
• Gene ID (NCBI): 8558
• RRID: AB_2878356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase 10 (CDK10) is a Cdc2-related kinase that was discovered based on its homology to the Cdc2 PSTA1RE amino acid domain (PMID: 8208557). CDK10 plays a pivotal role in the regulation of fundamental cellular processes, including cell proliferation, transcription regulation, and cell cycle regulation. It partners with cyclin M to phosphorylate substrates such as ETS2 and PKN2 in order to modulate cellular growth. Initial reports have indicated that CDK10 may act as a tumor suppressor in breast cancer (PMID: 26392360). Additional studies have demonstrated tumor suppressive and oncogenic roles for CDK10 in malignancies such as hepatobiliary cancers, gastric cancer, glioma, nasopharyngeal carcinoma, and colorectal cancer(PMID: 22326270,PMID: 29512714, PMID: 23740091, PMID: 29845196)

Publications

Species	Application	Title
human	WB	Oncol Lett; miR-433 accelerates acquired chemoresistance of gallbladder cancer cells by targeting cyclin M.; Authors - Jianhua Yu
human	WB	BMB Rep; Downregulated CDK10 promotes cancer progression and radioresistance in lung cancer through activating the JNK/c-Jun signaling pathway; Authors - Chaojin Hong ; KD Validated