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CDK1 Recombinant antibody, PBS Only

CDK1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240937C5

Cat no : 84271-1-PBS

Synonyms

CDC2, 240937C5, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK1 Recombinant antibody (84271-1-RR)

    WB analysis using 84271-1-RR (same clone as 84271-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84271-1-RR (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84271-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84271-1-RR

    Affinity and Kinetic Characterization of 84271-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84271-1-RR against Human CDK1 were performed. The affinity constant is 34.3 nM.

Product Information

The immunogen of 84271-1-PBS is CDK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK1 fusion protein Ag29018
Full Name cell division cycle 2, G1 to S and G2 to M
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC014563
Gene Symbol CDK1
Gene ID (NCBI) 983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.