VE-cadherin/CD144 Polyclonal antibody
VE-cadherin/CD144 Polyclonal Antibody for WB, IHC, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 27956-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HUVEC cells, human placenta tissue |
Positive IHC detected in | human placenta tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HUVEC cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 8 publications below |
IHC | See 1 publications below |
IF | See 11 publications below |
Product Information
27956-1-AP targets VE-cadherin/CD144 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | VE-cadherin/CD144 fusion protein Ag27487 |
Full Name | cadherin 5, type 2 (vascular endothelium) |
Calculated Molecular Weight | 88 kDa |
Observed Molecular Weight | 120-140 kDa |
GenBank Accession Number | NM_001795 |
Gene Symbol | VE-cadherin |
Gene ID (NCBI) | 1003 |
RRID | AB_2918136 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).
Protocols
Product Specific Protocols | |
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WB protocol for VE-cadherin/CD144 antibody 27956-1-AP | Download protocol |
IHC protocol for VE-cadherin/CD144 antibody 27956-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment | ||
Bioengineering (Basel) Organotypic 3D Co-Culture of Human Pleura as a Novel In Vitro Model of Staphylococcus aureus Infection and Biofilm Development | ||
World J Diabetes Tongxinluo promotes endothelium-dependent arteriogenesis to attenuate diabetic peripheral arterial disease | ||
Front Cell Infect Microbiol Analysis of miRNAs Involved in Mouse Heart Injury Upon Coxsackievirus A2 Infection. | ||
Bioengineering (Basel) A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis | ||
J Hypertens Short-chain acyl-CoA dehydrogenase is a potential target for the treatment of vascular remodelling |