P-cadherin Monoclonal antibody

P-cadherin Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1G10F7

Cat no : 66295-1-Ig

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin

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Validation Data Gallery

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  WB analysis of A431 using 66295-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66295-1-Ig (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 66295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66295-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 66295-1-Ig

  1X10^6 A431 cells were stained with 0.20ug P-cadherin antibody (66295-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66295-1-Ig targets P-cadherin in WB, IHC, FC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: P-cadherin fusion protein Ag4758
• Full Name: cadherin 3, type 1, P-cadherin (placental)
• Calculated Molecular Weight: 829 aa, 91 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC041846
• Gene Symbol: CDH3
• Gene ID (NCBI): 1001
• RRID: AB_2881678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.