CDCA7 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDCA7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (2)

Cat no : 15249-1-AP

Synonyms

Protein JPO1, JPO1, Cell division cycle-associated protein 7

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Validation Data Gallery

• 

  WB analysis using 15249-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15249-1-AP (CDCA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15249-1-AP

  WB result of CDCA7 antibody (15249-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDCA7 transfected HeLa cells.

• 

  IP experiment of HEK-293 using 15249-1-AP

  IP result of anti-CDCA7 (IP:15249-1-AP, 4ug; Detection:15249-1-AP 1:2000) with HEK-293 cells lysate 1480 ug.

• 

  IHC staining of human colon cancer using 15249-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15249-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15249-1-AP

  Immunofluorescent analysis of HepG2 cells using 15249-1-AP (CDCA7 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HEK-293 cells, Raji cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

15249-1-AP targets CDCA7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDCA7 fusion protein Ag4297
• Full Name: cell division cycle associated 7
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC027966
• Gene Symbol: CDCA7
• Gene ID (NCBI): 83879
• RRID: AB_2878119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCA7, also named as JP01, participates in MYC-mediated cell transformation and apoptosis. It plays a role as transcriptional regulator.

Publications

Species	Application	Title
mouse	WB,IF	Nucleic Acids Res; The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS; Authors - Akeo Shinkai
mouse	WB	Sci Adv; CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses; Authors - Maja Vukic
human	WB	Proc Natl Acad Sci U S A; HELLS and CDCA7 comprise a bipartite nucleosome remodeling complex defective in ICF syndrome.; Authors - Christopher Jenness
human	WB	J Exp Med; Loss of ZBTB24 impairs nonhomologous end-joining and class-switch recombination in patients with ICF syndrome.; Authors - Angela Helfricht
		Hum Mol Genet; Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.; Authors - Haoyu Wu
human	WB	Bioorg Chem; Novel isatin-based hybrids as potential anti-rheumatoid arthritis drug candidates: Synthesis and biological evaluation; Authors - Jian Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

XIAOBING (Verified Customer) (11-05-2019)

CDCA7 antibody was used to stain the 293T cells transfected with human CDCA7 plasmid. Overexpressed CDCA7 (Red) was localized in the nucleus (DAPI was used to label the nucleus).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: 293T overexpressing human CDCA7

CDCA7 Antibody Immunofluorescence, validation (1:250 dilution) in 293T overexpressing human CDCA7 (Cat no:15249-1-AP)

FH

Guillaume (Verified Customer) (09-08-2019)

I used cells mutated for ZBTB24 which control CDCA7 expression to test the specificity of the antibody. In WB and IP, the signal seem to be lower than the expected size in kDa. This CDCA7 antibody is not very efficient in ChIP

• Applications: Western Blot, Immunoprecipitation, ChIP,
• Cell Tissue Type: Fibroblasts and Lymphoblastoid cells