CDC2L1 Polyclonal antibody, PBS Only

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  WB analysis using 28613-1-AP (same clone as 28613-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28613-1-AP (CDC2L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 28613-1-AP (same clone as 28613-1-PBS)

  WB result of CDC2L1 antibody (28613-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC2L1 transfected HeLa cells. This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28613-1-AP (same clone as 28613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28613-1-AP (same clone as 28613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 28613-1-AP (same clone as 28613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28613-1-AP (same clone as 28613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28613-1-AP (same clone as 28613-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 28613-1-AP (same clone as 28613-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CDC2L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 28613-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

28613-1-PBS targets CDC2L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC2L1 fusion protein Ag28743
• Full Name: cell division cycle 2-like 1 (PITSLRE proteins)
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_001787
• Gene Symbol: CDC2L1
• Gene ID (NCBI): 984
• RRID: AB_2918181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P21127
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)