CDC25C Monoclonal antibody, PBS Only

CDC25C Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2B6B12

Cat no : 66912-1-PBS

Synonyms

M-phase inducer phosphatase 3, M phase inducer phosphatase 3, EC:3.1.3.48, CDC25, 2B6B12

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Validation Data Gallery

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  WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

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Product Information

66912-1-PBS targets CDC25C in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC25C fusion protein Ag10240
• Full Name: cell division cycle 25 homolog C (S. pombe)
• Calculated Molecular Weight: 473 aa, 53 kDa
• Observed Molecular Weight: 46-53 kDa
• GenBank Accession Number: BC019089
• Gene Symbol: CDC25C
• Gene ID (NCBI): 995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.