CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H3A4

Cat no : 67145-1-Ig

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

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Validation Data Gallery

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  WB analysis of Sp2/0 using 67145-1-Ig

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67145-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 67145-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Jurkat using 67145-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDC25B antibody (67145-1-Ig, Clone: 1H3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Sp2/0 cells, Jurkat cells, U-937 cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67145-1-Ig targets CDC25B in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC25B fusion protein Ag27193
• Full Name: cell division cycle 25 homolog B (S. pombe)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC006395
• Gene Symbol: CDC25B
• Gene ID (NCBI): 994
• RRID: AB_2882444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.